Khmer culture, as developed and spread by the Khmer empire, has distinctive styles of dance, architecture and sculpture, which have strongly influenced neighbouring Laos and Thailand. Angkor Wat (Angkor means "city" and Wat "temple") is the best preserved example of Khmer architecture from the Angkorian era and hundreds of other temples have been discovered in and around the region. The Tuol Sleng Genocide Museum, the infamous prison of the Khmer Rouge, and Choeung Ek, one of the main Killing Fields are other important historic sites.

Bonn Om Teuk (Festival of Boat Racing), the annual boat rowing contest, is the most attended Cambodian national festival. Held at the end of the rainy season when the Mekong river begins to sink back to its normal levels allowing the Tonle Sap River to reverse flow, approximately 10% of Cambodia's population attends this event each year to play games, give thanks to the moon, watch fireworks, and attend the boat race in a carnival-type atmosphere. Popular games include cockfighting, soccer, and kicking a sey, which is similar to a hacky sack. Notable recent artistic figures include the singers Sinn Sisamouth, who introduced new musical styles to the country, and later Meng Keo Pichenda.

Rice, as in other South East Asian countries, is the staple grain, while fish from the Mekong and Tonle Sap also form an important part of the diet. The Cambodian per capita supply of fish and fish products for food and trade in 2000 was 20 kilograms of fish per year or 2 ounces per day per person. Some of the fish can be made into prahok (a Khmer delicacy) for longer storage. Overall, the cuisine of Cambodia is similar to that of its Southeast Asian neighbours. The cuisine is relatively unknown to the world compared to that of its neighbors Thailand and Vietnam, but has been described not as spicy as Thai cuisine and similar to other Southeast Asia cuisines.

Football (soccer) is one of the more popular sports, although professional organized sports are not as prevelant in Cambodia as in western countries due to the economic conditions. The Cambodia national football team managed fourth in the 1972 Asian Cup but development has slowed since the civil war. Western sports such as volleyball, bodybuilding, field hockey, rugby, and baseball are gaining popularity while traditional boat racing maintains its appeal as a national sport. Martial Arts is also practiced in Cambodia, the most popular being Pradal Serey, which is similar to Muay Thai and also considered a national sport. Other styles such as Karate, Kung Fu and Taekwondo are rapidly catching on.